Properties, production, and applications of camelid single-domain antibody fragments
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of Recombinant Camelid Single-Domain Antibody in Tobacco
Antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. A promising alternative to conventional antibodies are the heavy chain antibodies (VHH) of Camelidae having short length, high solubility and stability are preferred to other antibody derivatives. In this study, our goal was product...
متن کاملBiotechnological applications of recombinant single-domain antibody fragments
BACKGROUND Single-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several research and biotechnological applications. RESULTS The discovery of the single-domain antibody's potentials has stimulated their use in an increa...
متن کاملcloning and expression of recombinant camelid single-domain antibody in tobacco
antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. a promising alternative to conventional antibodies are the heavy chain antibodies (vhh) of camelidae having short length, high solubility and stability are preferred to other antibody derivatives. in this study, our goal was product...
متن کاملSortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities.
A generic site-specific conjugation method that generates a homogeneous product is of utmost importance in tracer development for molecular imaging and therapy. We explored the protein-ligation capacity of the enzyme Sortase A to label camelid single-domain antibody-fragments, also known as nanobodies. The versatility of the approach was demonstrated by conjugating independently three different...
متن کاملBeneficial properties of single-domain antibody fragments for application in immunoaffinity purification and immuno-perfusion chromatography.
We explored the possibility to apply single-domain antibodies from Camelidae for immunoaffinity purification of the ice structuring protein (ISP) from Lolium perenne, which modifies ice crystal growth and therefore has potential application in medicine, biotechnology, agriculture and (frozen) foods. Using phage display together with an appropriate selection method, a group of candidate fragment...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2007
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-007-1142-2